RAFT polymers for protein recognition
نویسندگان
چکیده
A new family of linear polymers with pronounced affinity for arginine- and lysine-rich proteins has been created. To this end, N-isopropylacrylamide (NIPAM) was copolymerized in water with a binding monomer and a hydrophobic comonomer using a living radical polymerization (RAFT). The resulting copolymers were water-soluble and displayed narrow polydispersities. They formed tight complexes with basic proteins depending on the nature and amount of the binding monomer as well as on the choice of the added hydrophobic comonomer.
منابع مشابه
A triple carboxylic acid-functionalized RAFT agent platform for the elaboration of well-defined telechelic 3-arm star PDMAc.
This communication describes the synthesis of a triple acid-functionalized RAFT agent and its use to prepare well-defined 3-arm star polymers of N,N-dimethylacrylamide (DMAc). A simple esterification reaction allowed the convenient integration of three electron-rich naphthalene recognition units on the RAFT agent platform and subsequently the elaboration of a naphthalene end-decorated telecheli...
متن کاملSite-specific conjugation of RAFT polymers to proteins via expressed protein ligation.
Site-specific protein conjugates with RAFT polymers were synthesized using expressed protein ligation. Stable micelles were formed from both linear block copolymer and Y-shaped conjugates.
متن کاملPolymer Fluoride Sensors Synthesized by RAFT Polymerization.
A series of polymer chemosensors containing naphthalimide signal moiety and imide recognition moiety for the selective detection of fluoride ions (F(-) ) were synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization. The polymer chemosensors display obvious absorption and fluorescence variations upon the addition of F(-) , which can be observed by both naked eyes an...
متن کاملبررسی ویژگیهای اکوفیزیولوژیک گیاه نعناع در دو نوع محیط کشت آکواپونیک Raft و پرلیت
Aquaponic is a combination of fish and plant cultivated in recirculating systems. Fish culture in closed recirculating systems causes organic waste accumulation in the system. If these metabolites are used for plant nutrition, they are not mere waste, but have economical value and however benefit the fish production systems. Most aquaponic systems are designed as perlite-filled media, thin laye...
متن کاملEnd group removal and modification of RAFT polymers
This paper describes both well-established routes and recent advances in the end group modification of polymers synthesised by reversible addition–fragmentation chain transfer (RAFT) polymerisation. The lability of the thiocarbonylthio group, which facilitates the RAFT mechanism, allows for ready postpolymerisation functionalisation of RAFT polymers by a number of techniques. In particular, end...
متن کامل